High Throughput Detection of Drug-Metabolizing Enzyme Polymorphisms by Allele-Specific Fluorogenic 5′ Nuclease Chain Reaction Assay
書誌事項
- タイトル別名
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- High Throughput Detection of Drug-Metabolizing Enzyme Polymorphisms by Allele-Specific Fluorogenic 5' Nuclease Chain Reaction Assay.
- High throughput detection of drug-metabolizing enzyme polymorphisms by fluorogenic 5′ nuclease chain reaction assay
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説明
We have developed an allele-specific fluorogenic 5' nuclease chain reaction assay for detecting polymorphisms in the following human drug-metabolizing enzyme genes : CYP2C9 (CYP2C9*2 and *3), CYP2C19(CYP2C19*2 and *3), CYP2D6 (CYP2D6*4, *10, *14, *18, and *21(C8)), N-acetyltransferase 2 (NAT2*5B, *6A, and *7B), thiopurine methyltransferase (TPMT*3C), and aldehyde dehydrogenase2 (ALDH2*2). This method is a marriage of two emerging technologies, the use of allele-specific amplification primers for target DNA and hybridization of the TaqMan probe. The TaqMan probe is labeled with both a fluorescent reporter dye and a quencher dye. Genotypes are separated according to the different threshold cycles of the wild-type and mutant primers. All assays are performed using a single thermocycling protocol. This genotyping method is rapid and highly sensitive and yields a high throughput. It could be applied toward automated large-scale genotyping.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 23 (10), 1131-1135, 2000
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679603251584
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- NII論文ID
- 110003639841
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3cXnt1Kqs70%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 5506450
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- PubMed
- 11041238
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可