Nitric Oxide Stimulates Elastin Expression in Chick Aortic Smooth Muscle Cells.
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- SUGITANI Hideki
- Department of Clinical Chemistry, Hoshi College of Pharmacy
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- WACHI Hiroshi
- Department of Clinical Chemistry, Hoshi College of Pharmacy
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- TAJIMA Shingo
- Department of Dermatology, National Defense Medical College
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- SEYAMA Yoshiyuki
- Department of Clinical Chemistry, Hoshi College of Pharmacy
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Nitric oxide (NO), an endothelium-dependent relaxing factor, regulates relaxation, proliferation, and migration of smooth muscle cells (SMCs) and most likely attenuates developing vascular disease such as atherosclerosis. We investigated whether or not NO is associated with regulation of aortic elasticity. S-Nitrosoglutathione (GSNO), a NO donor, stimulated tropoelastin synthesis in cultured SMCs during both the quiescent and proliferating phases. The stimulation of tropoelastin synthesis was dose-dependent within 1—100 nM. Maximum stimulation was detected by treatment with 100 nM GSNO for 24 h. 8-Bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP), an exogenous cyclic GMP analog, also upregulated tropoelastin synthesis. Tropoelastin and lysyl oxidase mRNA expression, as assessed by Northern blot analysis, was also stimulated by GSNO. Administration of KT5823, a cyclic GMP-dependent protein kinase inhibitor, inhibited the GSNO-induced tropoelastin synthesis. These results indicate that the stimulatory effects of GSNO are due to cyclic GMP dependent protein kinase (PKG) activation by NO. In conclusion, NO seems to enhance aortic elasticity via tropoelastin and lysyl oxidase upregulation.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 24 (5), 461-464, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679603320704
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- NII論文ID
- 110003638492
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXjt1alsbw%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 5763831
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- PubMed
- 11379760
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可