Calcium Response and FcεRI Expression in Bone Marrow-Derived Mast Cells Co-cultured with SCG Neurites
-
- Suzuki Akio
- Graduate School of Pharmaceutical Sciences, Nagoya City University
-
- Suzuki Ryo
- Graduate School of Pharmaceutical Sciences, Nagoya City University
-
- Furuno Tadahide
- Graduate School of Pharmaceutical Sciences, Nagoya City University
-
- Teshima Reiko
- National Institute of Health Science, Japan
-
- Nakanishi Mamoru
- Graduate School of Pharmaceutical Sciences, Nagoya City University
書誌事項
- タイトル別名
-
- Calcium Response and Fc.EPSILON.RI Expression in Bone Marrow-Derived Mast Cells Co-cultured with SCG Neurites
- Calcium Response and Fc イプシロン RI Expression in Bone Marrow Derived Mast Cells Co cultured with SCG Neurites
この論文をさがす
説明
Communication between nerves and mast cells is a prototypic demonstration of neuro-immune interaction. Numerous studies have shown that the stimulation of nerves (or addition of neurotransmitters) can evoke activation of mast cells, and that mast cell-derived mediators can influence neuronal activity. However, it is still unknown whether high affinity IgE receptors (FcεRI) themselves are involved directly in the communication between nerves and mast cells. In the present experiments, we used an in vitro co-culture approach comprising interaction between immune (bone marrow-derived mast cells, BMMCs) and nerve cells (superior cervical ganglia, SCG) to solve the above problem. We found that the intracellular calcium ion concentration ([Ca2+]i) increased much more in BMMCs after antigen (DNP7-BSA) stimulation when they were associated with SCG neurites in the co-culture system. But the [Ca2+]i in BMMCs was less increased when they were not associated with the neurites. Further, the in vitro co-culture approach of BMMCs with SCG neurites for 3 d showed the increases of FcεRI expression occurred on the plasma membranes of BMMCs which were attached to the neurites. On the contrary, N-cadherin molecules which localized on the interface between on the plasma membrane of BMMCs and SCG neurites did not increase with the co-culture for 3 d. All of these results indicated that co-culturing BMMCs with SCG neurites for 3 d promoted not only the calcium response but also the FcεRI expression in BMMCs.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 28 (10), 1963-1965, 2005
公益社団法人 日本薬学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679603357696
-
- NII論文ID
- 110003666373
-
- NII書誌ID
- AA10885497
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 7452811
-
- PubMed
- 16204955
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可