Exosome-like vesicles with dipeptidyl peptidase 4 in human saliva

DOI Web Site Web Site 被引用文献13件 参考文献71件

書誌事項

タイトル別名
  • Exosome-Like Vesicles with Dipeptidyl Peptidase IV in Human Saliva
  • - like vesicles with dipeptidyl peptidase IV in human saliva

この論文をさがす

説明

Saliva contains a large number of proteins that participate in the protection of oral tissue. We found, for the first time, small vesicles (30—130 nm in diameter) in human whole saliva. Vesicles from saliva were identified by electron microscopy after isolation by gel-filtration on Sepharose CL-4B. They resemble exosomes, which are vesicles with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We performed a biochemical characterization of these vesicles by amino acid sequence analysis and Western blot analysis. We found that they contain dipeptidyl peptidase IV (DPP IV), galectin-3 and immunoglobulin A, which have potential to influence immune response. The DPP IV in the vesicles was metabolically active in cleaving substance P and glucose-dependent insulinotropic polypeptide to release N-terminal dipeptides. Our results demonstrate that human whole saliva contains exosome-like vesicles; they might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.

収録刊行物

被引用文献 (13)*注記

もっと見る

参考文献 (71)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ