Exosome-Like Vesicles with Dipeptidyl Peptidase IV in Human Saliva
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- Ogawa Yuko
- Institute of Medicinal Chemistry, Hoshi University
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- Kanai-Azuma Masami
- Department of Anatomy, Kyorin University School of Medicine
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- Akimoto Yoshihiro
- Department of Anatomy, Kyorin University School of Medicine
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- Kawakami Hayato
- Department of Anatomy, Kyorin University School of Medicine
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- Yanoshita Ryohei
- Institute of Medicinal Chemistry, Hoshi University
Bibliographic Information
- Other Title
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- Exosome-like vesicles with dipeptidyl peptidase 4 in human saliva
- - like vesicles with dipeptidyl peptidase IV in human saliva
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Abstract
Saliva contains a large number of proteins that participate in the protection of oral tissue. We found, for the first time, small vesicles (30—130 nm in diameter) in human whole saliva. Vesicles from saliva were identified by electron microscopy after isolation by gel-filtration on Sepharose CL-4B. They resemble exosomes, which are vesicles with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We performed a biochemical characterization of these vesicles by amino acid sequence analysis and Western blot analysis. We found that they contain dipeptidyl peptidase IV (DPP IV), galectin-3 and immunoglobulin A, which have potential to influence immune response. The DPP IV in the vesicles was metabolically active in cleaving substance P and glucose-dependent insulinotropic polypeptide to release N-terminal dipeptides. Our results demonstrate that human whole saliva contains exosome-like vesicles; they might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 31 (6), 1059-1062, 2008
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679603728128
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- NII Article ID
- 110006684315
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 9508929
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed