Polychlorinated Biphenyls Activate Caspase-3-Like Death Protease in Vitro but Not in Vivo.

DOI Web Site Web Site PubMed 被引用文献1件 参考文献36件
  • LEE Sang-Han
    Biopotency Evaluation Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • YOUK Eun-Soo
    Biopotency Evaluation Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • JEON Young Jin
    Biopotency Evaluation Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • HAN Sang Bae
    Biopotency Evaluation Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • KIM Hyoung-Chin
    Biopotency Evaluation Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • KIM Hwan Mook
    Biopotency Evaluation Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB)

この論文をさがす

抄録

We prove here that serum albumin inhibits apoptosis induced by polychlorinated biphenyls (PCBs), confirming that serum albumin binds to PCB, and that the albumin-PCB complexes inhibit apoptosis in HL-60 cells. We found that PCB (50 μM) increased the activity of caspase-3-like protease when HL-60 cells, as well as splenocytes, were cultured in “serum-free medium.” Benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-fmk) inhibited apoptosis in cells cultured in the serum-free medium containing 50 μM PCB. To elucidate whether or not PCBs induce apoptosis in vivo, we examined apoptosis of splenocytes by administering PCB to ICR mice (100, 500, 1000 mg·kg-1·d-1) for 5 d and characterizing splenocytes. Interestingly, splenocytes treated with PCB did not show any changes characteristic of apoptosis. These results demonstrate that PCB activates the caspase-3-like death protease in vitro in serum-free medium, but does not induce apoptosis of splenocytes in vivo, suggesting that blood serum may mask the apoptosis induced by PCB.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (36)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ