Effect of Culture Conditions on the Biosynthesis of Gagaminine, a Potent Antioxidant from the Roots of Cynanchum wilfordii.
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- LEE Dae-Won
- Department of Biology, College of Natural Science, Dongguk University
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- KIM Chanhong
- Department of Biology, College of Natural Science, Dongguk University
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- LEE Dong-Ung
- Department of Biochemistry, College of Natural Science, Dongguk University
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抄録
Gagaminine, a steroidal alkaloid isolated from the roots of Cynanchum wilfordii, exhibited potent inhibitory effects on the aldehyde oxidase activity and lipid peroxidation. To find a possible means of mass production of this active component, which will be useful for animal tests, we synthesized it by an in vitro culture method using various growth conditions. Calli were induced from the explants of this medicinal plant and cultivated under culture conditions which varied in light, and the kinds and concentration of plant growth regulators. The production of gagaminine was found to be significantly higher in the dark than in the light. The best gagaminine content (0.960%) was obtained after cultivation of stems on the medium containing 2,4-dichlorophenoxyacetic acid (2,4-D, 2.0 mg/l) only. However, gagaminine was not detected by the mixture of 2,4-D and kinetin, while the mixtures of 2,4-D/zeatin and 2,4-D/6-benzylaminopurine produced a low content of gagaminine (<0.4%). In addition, suspension medium was much better for the formation of gagaminine than solid medium with an increase from 0.960 to 2.227% yield. These results suggest that gagaminine can be produced massively by in vitro culture using stems under the conditions of dark and 2,4-D on liquid medium.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 24 (12), 1451-1453, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679603958144
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- NII論文ID
- 110003638441
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXosl2rs7g%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 5995972
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- PubMed
- 11767123
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可