Enhancement of Cellular Adenosine Triphosphate Levels in PC12 Cells by Extracellular Adenosine.
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- Fujimori Hiroyuki
- Faculty of Pharmaceutical Sciences, Setsunan University
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- Yasuda Masahiro
- Faculty of Pharmaceutical Sciences, Setsunan University
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- Pan-Hou Hidemitsu
- Faculty of Pharmaceutical Sciences, Setsunan University
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説明
To elucidate the biological significance of extracellular adenine compounds, the effects of adenosine (Ado) on cellular levels of adenine compounds, especially adenosine triphosphate (ATP), in PC12 cells were studied. Ado and inosine but not adenosine 5′-monophosphate, adenosine 5′-diphosphate, ATP, guanosine, cytosine, thymidine, and uridine, significantly enhanced cellular ATP levels in PC12 cells in time- and dose-dependent manners. Various P1 receptor agonists of Ado did not enhance the ATP level. In addition, theophylline, an antagonist of P1 receptors, did not inhibit the Ado-evoked ATP enhancement. These results suggest that the Ado receptor is not involved in the augmentation of the cellular ATP level induced by Ado in PC12 cells. The ATP-enhancing effect of Ado was potentiated by dipyridamole, an inhibitor of Ado uptake, or coformycin, an inhibitor of Ado deaminase. The effect of Ado on the ATP level was also observed when PC12 cells were incubated in glucose-free medium. Together these results suggest that enhancement of cellular ATP levels in PC12 cells by extracellular Ado might be acceleration of ATP synthesis through the Ado salvage system using hypoxanthine-guanine phosphoribosyltransferase rather than Ado kinase since 5′-iodotubercidin, an inhibitor of Ado kinase, had no effect on the enhancement elicited by Ado.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 25 (3), 307-311, 2002
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679604322944
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- NII論文ID
- 110003638692
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD38XkslWltrg%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 6263078
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- PubMed
- 11913523
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可