Functional Characterization of Serotonin Receptors in Rat Isolated Aorta.
-
- Villazón María
- Department of Pharmacology, Campus Sur s/n, School of Pharmacy
-
- Padín Juan Fernando
- Department of Pharmacology, Campus Sur s/n, School of Pharmacy
-
- Cadavid María Isabel
- Department of Pharmacology, Campus Sur s/n, School of Pharmacy
-
- Enguix María José
- Department of Pharmacology, Campus Sur s/n, School of Pharmacy
-
- Tristán Helena
- Department of Pharmacology, Campus Sur s/n, School of Pharmacy
-
- Orallo Francisco
- Department of Pharmacology, Campus Sur s/n, School of Pharmacy
-
- Loza María Isabel
- Department of Pharmacology, Campus Sur s/n, School of Pharmacy
この論文をさがす
説明
Interactions of serotonin (5-HT) with different specific 5-HT receptors that can coexist in the same blood vessel sometimes generate opposite effects. The aim of this study was to characterize the functional role of previously described mRNAs for 5-HT receptors in the rat aorta (5-HT2A, 5-HT2B, 5-HT1B, 5-HT7) as well as to study the known 5-HT2A receptor-mediated constrictor response and investigate the influences of endothelium and preconstriction on the tissue in that response. A slight endothelium- and concentration-dependent relaxant effect was observed for 5-HT in aorta precontracted with either 5 μM phenylephrine (PE) or 1 μM prostaglandin F2α (PGF2α) in the presence of 0.3 μM ketanserin. EC50 values for 5-HT and α-methyl-5-HT relaxant responses after PE were 43.10±4.00 and 57.11±8.01 nM, respectively. pKB values for antagonists cyproheptadine and rauwolscine were 8.92±0.22 and 7.15±0.12, respectively. In nonprecontracted tissues, the contractile potency of 5-HT was higher in the absence of endothelium (EC50, μM): 2.60±0.28 and 4.12±0.21 in the absence and in the presence of endothelium, respectively. The differences were statistically significant (p<0.05). In precontracted tissues, the differences in EC50 values (2.22±0.40 and 4.65±0.60 μM without and with endothelium, respectively) were also statistically significant (p<0.05). pKB values for the 5-HT2A antagonist ketanserin were similar under all conditions tested. In conclusion, under our experimental conditions there are two functional 5-HT receptors in rat aorta: 5-HT2A contractile receptor in smooth muscle and a high-affinity relaxant receptor that mediates a very slight response and the pharmacology of which could be compatible with an endothelial 5-HT2B receptor.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 25 (5), 584-590, 2002
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679604498944
-
- NII論文ID
- 110003638719
-
- NII書誌ID
- AA10885497
-
- COI
- 1:CAS:528:DC%2BD38Xkslyrtbg%3D
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 6154949
-
- PubMed
- 12033497
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可