The Anti-inflammatory Effect of Gigukjiwhangwhangami through the Inhibition of Nuclear Factor-κB Activation in the Peripheral Blood Mononuclear Cells of Patients with Cerebral Infarction

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  • Kim Su-Jin
    College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University College of Pharmacy, Wonkwang University
  • Jeong Hyun-Ja
    College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University
  • Moon Phil-Dong
    College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University
  • Lee Kang-Min
    Division of Biological Sciences, College of Natural Science, Chonbuk National University
  • Lee Min-Gu
    Professional Graduate School of Oriental Medicine, Wonkwang University
  • Moon Byung-Soon
    Professional Graduate School of Oriental Medicine, Wonkwang University
  • An Nyeon-Hyoung
    College of Pharmacy, Wonkwang University
  • Hong Seung-Heon
    College of Pharmacy, Wonkwang University
  • Na Ho-Jeong
    College of Pharmacy, Woosuk University
  • Jeon Hoon
    College of Pharmacy, Woosuk University
  • Kim Hyung-Min
    College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University
  • Um Jae-Young
    College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University

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タイトル別名
  • The Anti-inflammatory Effect of Gigukjiwhangwhangami through the Inhibition of Nuclear Factor-.KAPPA.B Activation in the Peripheral Blood Mononuclear Cells of Patients with Cerebral Infarction
  • Anti inflammatory Effect of Gigukjiwhangwhangami through the Inhibition of Nuclear Factor カッパ B Activation in the Peripheral Blood Mononuclear Cells of Patients with Cerebral Infarction

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抄録

The Korean genuine medicine “Gigukjiwhangwhangami (GJWGM)” has long been used for various cerebrovascular diseases. However, the exact mechanism that accounts for the anti-inflammatory effect of GJWGM is not completely understood. The aim of the present study is to elucidate how GJWGM modulates the inflammatory reaction in lipopolysaccaride (LPS)-stimulated peripheral mononuclear cells from patients with cerebral infarction. Production of cytokine was measured by the ELISA and RT-PCR method. The level of nuclear factor-kappaB (NF-κB)/Rel A protein and NF-κB DNA binding activity were determined by the Western blot analysis and TF-EIA method. We showed that GJWGM inhibited the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1α, IL-6, and IL-8 induced by LPS in dose dependent manner (p<0.05). Maximal inhibition rate of TNF-α, IL-1β, IL-6 and IL-8 production by GJWGM was about 54.34%, 41.37%, 44.04%, and 54.46%, respectively. GJWGM inhibited the TNF-α and IL-8 mRNA expression. In addition, we showed that the inhibitory mechanism of GJWGM is through the suppression of NF-κB pathway. Our study suggests that an important molecular mechanism by GJWGM reduce inflammation, which may explain its beneficial effect in the regulation of inflammatory reactions.

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