cDNA Cloning of the Sry-Related Gene Sox6 from Rat with Tissue-Specific Expression.
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- Narahara Masanori
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Yamada Akihisa
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Hamada-Kanazawa Michiko
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Kawai Yuichi
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Miyake Masaharu
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this cDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectively. The leucine-zipper and HMG-box motif were almost completely conserved between these homologues. The expression of Sox6 was determined in rat by Northern hybridization and Real-time quantitative reverse transcription (RT)-PCR. rSox6 (rat Sox6) was specifically expressed in the neonatal brain and adult testis with Northern blotting. Real-time quantitative RT-PCR for the determination of Sox6 mRNA was examined. The rSox6 was expressed in the neonatal brain and adult testis as well as by Northern blotting and also expressed in the adult eyeball and slightly in the ovary.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 25 (6), 705-709, 2002
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679604566656
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- NII論文ID
- 110003638743
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD38XkvV2hu7g%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 6174897
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- PubMed
- 12081133
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可