Effects of Hop Extracts on Nasal Rubbing and Sneezing in BALB/c Mice

Takubo Miho, Inoue Toshio, Jiang Shuishi, Tsumuro Tae, Ueda Yuhki, Yatsuzuka Rie, Segawa Shuichi, Watari Junji, Kamei Chiaki

doi:10.1248/bpb.29.689

The effects of hop extracts (Humulus lupulus L.) on histamine release from rat peritoneal mast cells and human basophilic KU812 cells were studied. Hop water extract (HWE) and XAD-4 50% methanol fraction of HWE (MFH) inhibited histamine release from rat mast cells induced by compound 48/80 at concentrations of 100 and 10 μg/ml, respectively. Almost the same findings were observed with A23187-induced histamine release from KU812 cells. Next, we studied the effects of hop extracts on antigen-induced nasal rubbing and sneezing in sensitized BALB/c mice. HWE caused a significant inhibition of nasal rubbing and sneezing at a dose of 500 mg/kg. MFH also inhibited nasal rubbing and sneezing dose-dependently. A significant difference was observed from 100 mg/kg in nasal rubbing and 200 mg/kg in sneezing. The effects of both extracts became clear after repeated administration. HWE and MFH significantly inhibited both nasal rubbing and sneezing, respectively, after consecutive treatment for 15 d at smaller doses compared with single administration. This finding indicates that the active component of hop is included in MFH, which was absorbed to Amberlite XAD-4 and eluted with 50% methanol. These results clearly demonstrated that hop extracts may be effective in the relief of symptoms of allergic rhinitis.

Effects of Hop Extracts on Nasal Rubbing and Sneezing in BALB/c Mice

Search this article

Description

Journal

Citations (13)*help

References(27)*help

Keywords

Details 詳細情報について

Export

Report a problem