Effect of Geometrical Structure on the Biodegradation of a Three-Dimensionally Perforated Porous Apatite/Collagen Composite Bone Cell Scaffold

Hamada Hideyuki, Ohshima Hiroyuki, Ito Atsuo, Higuchi William I., Otsuka Makoto

doi:10.1248/bpb.33.1228

A Biodegradable artificial bone with inter-connective pores was prepared using a self-setting apatite/collagen composite cement as a cell scaffold for bone regenerative medicine, and investigated as to biocompatibility by X-ray computed tomography (CT) after its implantation into rats. Blocks (APN, APC and ACC) of apatite cement, apatite cement with continuous holes, and apatite/collagen composite cement with continuous holes were prepared. The APC and ACC blocks had 16 (8×2) interconnecting holes 500 μm in diameter. After the APN, APC, and ACC blocks were implanted in the back of the rats, X-ray CT images were measured every week. Before and after implantation, powder X-ray diffraction profiles of APN, APC and ACC showed diffraction patterns of hydroxyapatite with low crystallinity. Changes in the volume, inorganic content and density of the blocks in the rats were evaluated based on X-ray CT images. The volume and inorganic content of ACC decreased continuously at a constant rate. In contrast, the volume and inorganic content of APN and APC didn't show major changes. After implantation, the absorption of X-rays by ACC decreased with time. This suggested that the block was bioabsorbed significantly with time. In contrast, the absorption of APC and APN did not decrease, indicating that the blocks were not bioabsorbed.

Effect of Geometrical Structure on the Biodegradation of a Three-Dimensionally Perforated Porous Apatite/Collagen Composite Bone Cell Scaffold

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