Determination of 14 Chemical Constituents in the Traditional Chinese Medicinal Preparation Huangqin-Tang by High Performance Liquid Chromatography.

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  • ZUO Feng
    Institute of Chinese Material Medica, China Academy of Traditional Chinese Medicine
  • ZHOU Zhong-Ming
    Institute of Chinese Material Medica, China Academy of Traditional Chinese Medicine
  • LIU Mei-Lan
    Institute of Chinese Material Medica, China Academy of Traditional Chinese Medicine

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The high performance liquid chromatographic (HPLC) method for the identification and determination of baicalin (BG), wogonoside (WG), oroxylin-A-glucoside (OG), baicalein (B), wogonin (W), orxylin-A (O), paeoniflorin (PF), glycyrrhizic acid (GL), glycyrrhetinic acid (GA), liquiritin (LG), isoliquirition (ILG), liquiritigenin (L), isoliquiritigenin (IL) and ononin (ON) in Huangqin-Tang (黄苓湯) was established. The samples were separated with a Wakosil C18 column (4.6×150 mm) by linear gradient elution using A (MeOH-HAC 100:1, v/v)-B (Water-HAC 100:1, v/v) (0 min, 30:70; 15 min, 40:60; 30 min, 60:40; 45 min, 80:20; 60 min, 100:0) as the mobile phase at a flow-rate of 1.0 ml/min. The detection was by diode-array UV/Vis detector (DAD), and the wavelength was set at the range of 200—400 nm. Satisfactory results were obtained within 60 min for the simultaneous determination of the 14 constituents. The repeatability (RSD) of the method was generally less than 2% (n=5, interday and intraday). The recovery of BG was 96.9±1.71, WG was 98.9±2.99, PF was 99.7±0.52, LG was 95.3±2.67, GL was 96.7±3.44, and GA was 94.8±4.16, respectively.

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