Granulomatous Tissue Formation of Shikon and Shikonin by Air Pouch Method.

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  • SAKAGUCHI Ikuyo
    Institute of Cosmetic Sciences, Club Cosmetics Co., Ltd. Department of Anatomy, Osaka City University Medical School
  • TSUJIMURA Masae
    Institute of Cosmetic Sciences, Club Cosmetics Co., Ltd.
  • IKEDA Norikazu
    Institute of Cosmetic Sciences, Club Cosmetics Co., Ltd.
  • MINAMINO Miki
    Institute of Cosmetic Sciences, Club Cosmetics Co., Ltd.
  • KATO Yoshiko
    Institute of Cosmetic Sciences, Club Cosmetics Co., Ltd.
  • WATABE Kazuhito
    Department of Microbiology for Public Health, Faculty of Pharmaceutical Sciences, Setsunan University
  • YANO Ikuya
    Japan BCG Laboratory
  • KANEDA Kenji
    Department of Anatomy, Osaka City University Medical School

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Abstract

“The extract of shikon” (SK) and shikonin play important roles in the development of granulomatous tissue formation. To reveal the augmenting effect of SK or shikonin on vascular endothelial growth factor (VEGF) production and neovascularization, we investigated murine granulomatous tissue induced by SK and shikonin, comparing them to pouches in which trehalose 6,6'-dimycolate (TDM) was injected. The development of granulomatous tissue formation was evaluated by the wet weight of pouch walls. At day 5 and 7 after SK and shikonin injection, prominent granulomatous tissue formation was detected. Histological observations on the development of granulomatous tissue showed that the pouch was formed in the submuscular connective tissue and necrotic tissue directly facing the cavity and granulomatous tissue developed in the connective tissue. At day 1, VEGF-positive neutrophils accumulated in the pouch wall. Granulomatous tissue formation and neovascularization by injection of SK or shikonin was not more prominent than TDM. However, the present results indicate that SK and shikonin induce neovascularization in granulomatous tissue.

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