Hepatoprotective Effects of Phloridzin on Hepatic Fibrosis Induced by Carbon Tetrachloride against Oxidative Stress-Triggered Damage and Fibrosis in Rats

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  • Deng Gaigai
    Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University
  • Wang Junzhi
    Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University
  • Zhang Qiaoyin
    Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University
  • He Haibo
    Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University
  • Wu Fangfang
    Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University
  • Feng Tianyan
    Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University
  • Zhou Jigang
    Traditional Chinese Medicine Clinical Hospital of China, Three Gorges University
  • Zou Kun
    Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University
  • Hattori Masao
    Division of Metabolic Engineering, Institute of Natural Medicine, University of Toyama

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Abstract

The present study was to study the hepatoprotective effects of phloridzin (PHL) on hepatic fibrosis induced by carbon tetrachloride (CCl4) in rats, on the basis of this investigation, the possible mechanism of PHL was elucidated. Male Sprague Dawley (SD) rats were randomly divided into six groups: control, model, PHL-L, PHL-M, PHL-H and colchine. All rats except control group were intraperitoneally injected with CCl4, and control rats were injected with olive oil, twice a week for eight weeks. At the same time, the rats were orally given homologue drugs once a day, respectively. Hepatoprotective effects of PHL were evaluated by liver weight indexes, biochemical values, total antioxidant capacity and total-superoxide dismutase, histopathological observations, hepatic fibrosis, and the hepatic fibrosis relative gene and protein expressions. PHL significantly improved hepatic function; remarkably decreased serum hyaluronic acid (HA), transforming growth factor-β1 (TGF-β1), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and liver tissues hydroxyproline, malondialdehyde (MDA) levels, increased glutathione peroxidase (GSH-Px), total-antioxygen capacity (T-AOC) and total-superoxide dismutase (T-SOD) contents of liver tissues; Real-time polymerase chain reaction (PCR) and immunohisto-chemical results showed PHL might markedly reverse the up-regulated mRNA and protein expressions of the α-smooth muscle actin (SMA), TGF-β1 and tissue inhibitor of metalloproteinase-1 (TIMP1), up-regulate the matrix metalloproteinase-1 (MMP1) mRNA and protein expressions. Histopathological observations provided supportive evidence for biochemical analyses and the hepatic fibrosis relative gene and protein expressions, and with the dose of PHL increasing, the aforesaid improvement became more and more strong. The studies demonstrated that PHL exerted beneficially hepatoprotective effects on hepatic fibrosis induced by CCl4, mainly enhancing antioxidant capacity of liver organizations, reduce the level of lipid peroxidation induced by CCl4, and protect hepatocyte membranes from damage, and alleviate hepatic fibrosis.

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