Binding Properties of Antimicrobial Agents to Lipid Membranes Using Surface Plasmon Resonance
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- Kinouchi Hiroki
- Pharmaceutical Research Division, Shionogi & Co., Ltd. Department of Biochemistry, Kobe Pharmaceutical University
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- Arimoto Hirokazu
- Graduate School of Life Sciences, Tohoku University
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- Nishiguchi Kenzo
- Pharmaceutical Research Division, Shionogi & Co., Ltd.
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- Oka Masako
- Pharmaceutical Research Division, Shionogi & Co., Ltd.
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- Maki Hideki
- Pharmaceutical Research Division, Shionogi & Co., Ltd.
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- Kitagawa Hiroshi
- Department of Biochemistry, Kobe Pharmaceutical University
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- Kamimori Hiroshi
- Pharmaceutical Research Division, Shionogi & Co., Ltd.
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抄録
In the present study, we examined the interaction of antimicrobial agents with four model lipid membranes that mimicked mammalian cell membranes and Gram-positive and -negative bacterial membranes and analyzed the binding kinetics using our surface plasmon resonance (SPR) technique. The selective and specific binding characteristics of antimicrobial agents to the lipid membranes were estimated, and the kinetic parameters were analyzed by application of a two-state reaction model. Reproducible analysis of binding kinetics was observed. Vancomyicn, teicoplanin, erythromycin, and linezolid showed little interaction with the four lipid membranes in the SPR system. On the other hand, vancomycin analogues showed interaction with the model lipid membranes in the SPR system. The selective and specific binding characteristics of vancomycin analogues to the lipid membranes are discussed based on data for in vitro antibacterial activities and our data on the binding affinity of the D-alanyl-D-alanine terminus of a pentapeptide cell wall obtained by SPR. The mechanism of antibacterial activity against Staphylococcus aureus and vancomycin-resistant enterococci could be evaluated using the binding affinity obtained with our SPR techniques. The results indicate that the SPR method could be widely applied to predict binding characteristics, such as selectivity and specificity, of many antimicrobial agents to lipid membranes.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (8), 1383-1389, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679608898688
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- NII論文ID
- 130004677547
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cbos1Kiuw%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025610215
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- PubMed
- 25087959
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可