Serotonin Suppresses β-Casein Expression via Inhibition of the Signal Transducer and Activator of Transcription 5 (STAT5) Protein Phosphorylation in Human Mammary Epithelial Cells MCF-12A
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- Chiba Takeshi
- Department of Clinical Pharmaceutics and Pharmacy Practice, School of Pharmacy, Iwate Medical University
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- Kimura Soichiro
- Department of Hospital Pharmacy, Faculty of Pharmaceutical Sciences, Josai University
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- Takahashi Katsuo
- Department of Clinical Pharmaceutics and Pharmacy Practice, School of Pharmacy, Iwate Medical University
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- Morimoto Yasunori
- Department of Hospital Pharmacy, Faculty of Pharmaceutical Sciences, Josai University
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- Sanbe Atsushi
- Department of Pharmacotherapeutics, School of Pharmacy, Iwate Medical University
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- Ueda Hideo
- Department of Hospital Pharmacy, Faculty of Pharmaceutical Sciences, Josai University
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- Kudo Kenzo
- Department of Clinical Pharmaceutics and Pharmacy Practice, School of Pharmacy, Iwate Medical University
書誌事項
- タイトル別名
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- Serotonin Suppresses β-Casein Expression <i>via</i> Inhibition of the Signal Transducer and Activator of Transcription 5 (STAT5) Protein Phosphorylation in Human Mammary Epithelial Cells MCF-12A
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抄録
Serotonin (5-hydroxytryptamine; 5-HT) has an important physiological role in controlling lactation, namely, milk volume homeostasis, within mammary glands. The objectives of this study were to evaluate whether exogenous 5-HT can suppress β-casein expression, a differentiation marker, produced in human mammary epithelial cells, and to determine whether 5-HT can attenuate β-casein signaling via the prolactin (PRL) receptor (PRLr) and Janus kinase 2/signal transducer and activator of transcription 5 (STAT5) pathway. PRL treatment increased the mRNA level of β-casein in the MCF-12A human mammary epithelial cell line, and the highest level occurred at days 7 and 14 of culture. In contrast, PRLr expression was not affected significantly by PRL treatment. PRL treatment in MCF-12A cells increased levels of β-casein and phosphorylated STAT5 (pSTAT5) proteins in a concentration-dependent manner, with a slight increase of STAT5 protein. β-Casein expression was inhibited by 0.1 mM 5-HT in a time-dependent manner. Additionally, treatment with 0.1 mM 5-HT for 72 h decreased protein levels of β-casein and pSTAT5, with a slight decrease in STAT5 levels. These results suggest that exogenous 5-HT can inhibit STAT5 phosphorylation, resulting in a decrease in β-Casein expression. In conclusion, we showed that exogenous 5-HT decreased β-casein expression in MCF-12A human mammary epithelial cells, and that 5-HT was responsible for inhibiting phosphorylation of STAT5, resulting in a decline in lactational function.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (8), 1336-1340, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679608904960
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- NII論文ID
- 130004677543
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cbos1Kitw%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025610047
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- PubMed
- 25087955
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可