RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method

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  • Tawaraya Yuri
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University
  • Hyodo Mamoru
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University
  • Ara Mst Naznin
    Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University
  • Yamada Yuma
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University
  • Harashima Hideyoshi
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University

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The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2′-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.

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