Vascular Endothelial Growth Factor Modulates Voltage-Gated Na+ Channel Properties and Depresses Action Potential Firing in Cultured Rat Hippocampal Neurons

DOI Web Site Web Site PubMed 参考文献15件
  • Sun Guang-chun
    Department of Pharmacy, The Fifth People’s Hospital of Shanghai, Fudan University
  • Ma Yuan-yuan
    State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University

この論文をさがす

説明

Vascular endothelial growth factor (VEGF), an angiogenic factor, was found to modulate synaptic plasticity by affecting K+ and Ca2+ channels and protect neuron from death by depressing glutamatergic transmission. However, whether VEGF also modulates neuronal activity through modulating voltage-gated Na+ channels (VGSCs), a main determinant of neuronal excitability, we observed the effects of VEGF on Na+ channel properties and function on cultured rat hippocampal neurons through whole-cell patch-clamp recording. We found that VEGF decreased the Na+ channel excitability by shifting the voltage-dependence of steady-state inactivation to more hyperpolarized direction, and increasing the time constants of recovery from inactivation without significantly affecting the activation process. The effect of VEGF on Na+ channel steady-state inactivation was inhibited by the specific VEGF Flk-1 receptor antagonist SU1498, but was not affected by protein kinase C (PKC)-activator 1-oleoyl-2-acetyl-sn-glycerol (OAG). Furthermore, the inhibition of Na+ currents by VEGF was frequency-dependent. In addition, the frequency of neuron firing evoked by current injection was reversibly depressed by VEGF. Therefore, our results suggest a potential role of VGSCs in the modulation of VEGF on neuronal excitability.

収録刊行物

参考文献 (15)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ