Protective Effects of Fluvoxamine against Ischemia/Reperfusion Injury in Isolated, Perfused Guinea-Pig Hearts
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- Muto Tatsuya
- College of Pharmacy, Kinjo Gakuin University Meitetsu Hospital Pharmacy
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- Usuda Haruki
- College of Pharmacy, Kinjo Gakuin University
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- Yamamura Aya
- College of Pharmacy, Kinjo Gakuin University
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- Yoshida Koji
- College of Pharmacy, Kinjo Gakuin University
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- Ohashi Ai
- College of Pharmacy, Kinjo Gakuin University
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- Mitsui-Saitoh Kumiko
- College of Pharmacy, Kinjo Gakuin University Faculty of Sports and Health Science, Nagoya Gakuin University
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- Sakai Junichi
- College of Pharmacy, Kinjo Gakuin University Faculty of Sports and Health Science, Nagoya Gakuin University
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- Sugimoto Yumi
- Laboratory of Pharmacology, Department of Clinical Pharmacy, Yokohama College of Pharmacy
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- Mizutani Hideki
- College of Pharmacy, Kinjo Gakuin University
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- Nonogaki Tsunemasa
- College of Pharmacy, Kinjo Gakuin University
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- Hotta Yoshihiro
- College of Pharmacy, Kinjo Gakuin University Department of Pharmacology, Aichi Medical University School of Medicine
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Abstract
Serotonin (5-hydroxytryptamine; 5-HT) is known to be activated during ischemia–reperfusion and triggers contractile dysfunction and pathological apoptosis. Here, the beneficial effects of the selective serotonin reuptake inhibitor (SSRI) fluvoxamine was demonstrated on ischemia–reperfusion injury in guinea-pig hearts perfused using the Langendorff technique. The recovery (%) of left ventricular developed pressure (LVDP) by fluvoxamine (5×10−8 M) was 95.4% (control: 32%), which was consistent with the inhibition of mitochondrial Ca2+([Ca2+]m) uptake induced by changes in the Ca2+ content and acidification of the perfusate, and similar to reperfusion following global ischemia in Langendorff-perfused hearts. Fluvoxamine inhibited the increase in [Ca2+]m induced by changes in the Ca2+ content of the perfusate in perfused preparations of mitochondria, which was similar to the results obtained with the mitochondrial permeability transition pore (MPTP) opener atractyroside. The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)-positive cells were significantly less in fluvoxamine-treated hearts than in control hearts, with decreases in caspase-3 activity. These results suggest that SSRI inhibits opening of the MPTP by preventing [Ca2+]m overload-induced apoptosis related to the endogenous accumulation of 5-HT in ischemia–reperfusion hearts.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 37 (5), 731-739, 2014
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679609175808
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- NII Article ID
- 130004147319
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- NII Book ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cnpsVClsA%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 025419826
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- PubMed
- 24789996
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed