Grape Seed Proanthocyanidin Extracts Enhance Endothelial Nitric Oxide Synthase Expression through 5′-AMP Activated Protein Kinase/Surtuin 1–Krüpple Like Factor 2 Pathway and Modulate Blood Pressure in Ouabain Induced Hypertensive Rats

DOI Web Site Web Site PubMed 4 Citations 35 References
  • Cui Xiaopei
    Department of Geriatrics, Qilu Hospital of Shandong University
  • Liu Xiangju
    Department of Geriatrics, Qilu Hospital of Shandong University
  • Feng Hua
    Department of Gastroenterology, Shandong Provincial Hospital
  • Zhao Shaohua
    Department of Geriatrics, Qilu Hospital of Shandong University
  • Gao Haiqing
    Department of Geriatrics, Qilu Hospital of Shandong University

Bibliographic Information

Other Title
  • Grape Seed Proanthocyanidin Extracts Enhance Endothelial Nitric Oxide Synthase Expression through 5'-AMP Activated Protein Kinase/Surtuin 1–Krupple Like Factor 2 Pathway and Modulate Blood Pressure in Ouabain Induced Hypertensive Rats
  • Grape seed proanthocyanidin extracts enhance endothelial nitric oxide synthase expression through 5’-AMP activated protein kinase/Surtuin 1-Krüpple like factor 2 pathway and modulate blood pressure in ouabain induced hypertensive rats

Search this article

Description

Grape seed proanthocyanidin extracts (GSPE) belonging to polyphenols, possess various biological effects including anti-inflammation, anti-oxidant, anti-aging, anti-atherosclerosis, etc. GSPE is potential in regulating endothelial function. However, the underlying mechanism is not clear yet. In this study, by small interfering RNA (siRNA) knocking down, we proved that GSPE increase endothelial nitric oxide synthase (eNOS) expression in human umbilical vessel cells (HUVECs) in vitro, which was attributed to its transcription factor Krüpple like factor 2 (KLF2) induction. Furthermore, GSPE activate 5′-AMP activated protein kinase (AMPK) and increase surtuin 1 (SIRT1) protein level, critical for KLF2 induction. We also illuminated the role of GSPE in hypertension treatment. By chronic administration of GSPE in ouabain induced hypertensive rats model, we access the effect of GSPE on blood pressure regulation and the possible mechanisms involved. After 5 weeks feeding, GSPE significantly block the ouabain induced blood pressure increase. The aortic NO production impaired by ouabain was improved. In conclusion, GSPE increase eNOS expression and NO production in an AMPK/SIRT1 dependent manner through KLF2 induction, and attenuate ouabain induced hypertension.

Journal

Citations (4)*help

See more

References(35)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top