<i>Clostridium perfringens</i> TpeL Induces Formation of Stress Fibers <i>via</i> Activation of RhoA-ROCK Signaling Pathway
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- Nagahama Masahiro
- Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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- Ohkubo Akiko
- Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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- Kinouchi Yoshihito
- Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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- Kobayashi Keiko
- Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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- Miyamoto Kazuaki
- Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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- Takehara Masaya
- Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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- Sakurai Jun
- Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
Bibliographic Information
- Other Title
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- Clostridium perfringens TpeL Induces Formation of Stress Fibers via Activation of RhoA-ROCK Signaling Pathway
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Abstract
Clostridium perfringens TpeL belongs to a family of large clostridial glucosylating cytotoxins. TpeL modifies Rac1 and Ras subfamily proteins. Herein we report TpeL-induced formation of stress fibers via RhoA-Rho kinase (ROCK) signaling. A recombinant protein (TpeL1–525) derived from the TpeL N-terminal catalytic domain in the presence of streptolysin O (SLO) induced the formation of actin stress fibers in Madin–Darby canine kidney (MDCK) cells in a dose-dependent manner. The RhoA/ROCK pathway is known to control the formation of stress fibers. We examined the role of the RhoA/ROCK pathway in TpeL-induced formation of stress fibers. TpeL1–525-induced formation of stress fibers was inhibited by the ROCK inhibitor, Y27632 and Rho protein inhibitor, C3 transferase. TpeL1–525 activated RhoA and ROCK in a dose-dependent manner. C3 transferase blocked TpeL1–525-induced activation of RhoA and ROCK whereas Y27632 inhibited TpeL-induced activation of ROCK. These results demonstrate for the first time that TpeL induces the formation of stress fibers by activating the RhoA/ROCK signaling pathway.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 38 (5), 732-739, 2015
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679609577984
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- NII Article ID
- 130005068100
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 026355306
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- PubMed
- 25947919
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed