Involvement of Histaminergic System in the Anxiolytic-Like Activities of <i>Morus alba</i> Leaves in Mice
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- Lee Seungheon
- Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Science, Jeju National University
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- Kim Dong Hyun
- School of Clinical Sciences, University of Bristol
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- Lee Ji Hye
- Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University
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- Ko Eun Seong
- Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Science, Jeju National University
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- Oh Won Bo
- Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Science, Jeju National University
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- Seo Yong Taek
- Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University
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- Jang Young Pyo
- Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University
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- Ryu Jong Hoon
- Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University
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- Jung Ji Wook
- Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University
Bibliographic Information
- Other Title
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- Involvement of Histaminergic System in the Anxiolytic-Like Activities of Morus alba Leaves in Mice
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Abstract
The aim of this study was to identify the effects of 85% methanolic extract of Morus alba leaves (EMA), which is a traditional herb, in mice. The effects of EMA on the anxiolytic-like behaviour were studied using the elevated plus maze (EPM) and hole-board test. To elucidate the mode of action of the anxiolytic-like effects of EMA, the mice were subjected to the co-administration of EMA (200 mg/kg, per os (p.o.)) and either antagonist. EMA (at 200 or 400 mg/kg) significantly increased the percentages of time-spent in the open arms and entries into the open arms of the EPM versus vehicle-treated control group (p<0.05). Moreover, in the hole-board test, EMA (200 and 400 mg/kg) significantly increased the number of head-dips versus vehicle-treated control group (p<0.05). However, there were no changes in the locomotor activity and myorelaxant effects in any group compared with the vehicle-treated control group. In addition, the anxiolytic-like effects of EMA were abolished by thioperamide (10 mg/kg, intraperitoneally (i.p.)), which is a histamine H3 receptor antagonist. Moreover, results from reverse transcription polymerase chain reaction (RT-PCR) also revealed that the amygdalal histidine decarboxylase mRNA expression levels in EMA (200 mg/kg)-treated group were significantly higher than those in the vehicle-treated controls (p<0.05). These results suggest that EMA might prove to be an effective anxiolytic agent and that EMA acts via the histaminergic system in central nerve system.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 36 (11), 1692-1699, 2013
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679609728896
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- NII Article ID
- 130003361548
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- NII Book ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC3sbhvVCrug%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 024957494
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- PubMed
- 23965748
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed