Protective Effect of SUN N8075, a Free Radical Scavenger, against Excessive Light-Induced Retinal Damage in Mice

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  • Ojino Kazuki
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University
  • Shimazawa Masamitsu
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University
  • Ohno Yuta
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University
  • Otsuka Tomohiro
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University
  • Tsuruma Kazuhiro
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University
  • Hara Hideaki
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University

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Abstract

Although dry age-related macular degeneration (AMD) is one of the major causes of blindness, no effective therapies are developed. In this study, we investigated the effects of SUN N8075, a radical scavenger with neuroprotective properties, against light-induced retinal damage used as the model of dry AMD in mice. After dark adaption for 24 h, we exposed the mice at 8000 lx for 3 h. We evaluated the retinal damage by recording the electroretinagram (ERG) and measuring the thickness of outer nuclear layer (ONL) at 5 d after the light exposure. Retinal apoptotic cells were also detected by terminal deoxynucleotidyl transeferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining, and the expression of 8-hydroxy-2-deoxyguanosine (8-OHdG) as an index for oxidative stress at 48 h after exposure to light. In ERG measurement, the intraperitoneal administration of SUN N8075 at 30 mg/kg improved the retinal dysfunction induced by the excess light exposure. In the histological evaluation, SUN N8075 inhibited the reduction of ONL thickness. In addition, SUN N8075 decreased in both numbers of TUNEL- and 8-OHdG-positive cells in ONL. These findings suggest that the systemic administration of SUN N8075 has protective effects on excess light-induced photoreceptor degeneration, via inhibition of oxidative stress.

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