Evaluation of Aculeatin and Toddaculin Isolated from <i>Toddalia asiatica</i> as Anti-inflammatory Agents in LPS-Stimulated RAW264 Macrophages
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- Kumagai Momochika
- Japan Food Research Laboratories, Saito Laboratory Department of Chemistry, Graduate School of Science, Osaka City University
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- Watanabe Akio
- Research Institute for Biological Functions, Chubu University
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- Yoshida Izumi
- Japan Food Research Laboratories, Saito Laboratory
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- Mishima Takashi
- Japan Food Research Laboratories, Saito Laboratory
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- Nakamura Munetomo
- Japan Food Research Laboratories, Saito Laboratory
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- Nishikawa Keisuke
- Department of Chemistry, Graduate School of Science, Osaka City University
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- Morimoto Yoshiki
- Department of Chemistry, Graduate School of Science, Osaka City University
Bibliographic Information
- Other Title
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- Evaluation of Aculeatin and Toddaculin Isolated from Toddalia asiatica as Anti-inflammatory Agents in LPS-Stimulated RAW264 Macrophages
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Abstract
<p>Anti-inflammatory activity of aculeatin and toddaculin, which are coumarins with a similar structure isolated from Toddalia asiatica (L.) LAM., was evaluated using lipopolysaccharide (LPS)-stimulated RAW264 mouse macrophage cells. Both aculeatin and toddaculin significantly inhibited mRNA expression of inflammatory mediators and nitric oxide production. Furthermore, Toddaculin suppressed LPS-induced phosphorylation of p38 and extracellular signal-regulated kinase (ERK)1/2 and inhibited LPS-induced activation of nuclear factor-kappaB (NF-κB). However, aculeatin did not exhibit such effects, suggesting that aculeatin and toddaculin suppress LPS-induced inflammation of RAW264 cells via different mechanisms. The cellular uptake of these compounds was also evaluated. Toddaculin was detected in RAW264 cells after 4 and 24 h. However, aculeatin levels were not observed in RAW264 cells at all incubation intervals. These results indicate that de-epoxidation of a prenyl group can increase hydrophobicity of molecule and is thought to accelerate cellular uptake and/or interactions with the phospholipid bilayers of cell membranes.</p>
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 41 (1), 132-137, 2018
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679610975360
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- NII Article ID
- 130006301210
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 028739469
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- PubMed
- 29311475
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed