Tissue-Clearing Techniques Enable Three-Dimensional Visualization of Aerosolized Model Compound and Lung Structure at the Alveolar Scale
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- Togami Kohei
- Division of Pharmaceutics, Hokkaido Pharmaceutical University School of Pharmacy
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- Kitayama Anri
- Division of Pharmaceutics, Hokkaido Pharmaceutical University School of Pharmacy
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- Daisho Takayuki
- Division of Pharmaceutics, Hokkaido Pharmaceutical University School of Pharmacy
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- Wang Rui
- Division of Pharmaceutics, Hokkaido Pharmaceutical University School of Pharmacy
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- Tada Hitoshi
- Division of Pharmaceutics, Hokkaido Pharmaceutical University School of Pharmacy
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- Chono Sumio
- Division of Pharmaceutics, Hokkaido Pharmaceutical University School of Pharmacy
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Description
<p>In this study, we examined the usefulness of a tissue-clearing technique for the evaluation of the lung distribution of aerosolized drugs. An aerosol formulation of TexasRed dextran (70 kDa), a model compound of drug carrier for aerosolized drugs, was administered intrapulmonarily to mice using a MicroSprayer, and then DyLight 488-conjugated tomato lectin was administered intravenously to visualize general lung structure via the fluorescent labeling of alveolar and bronchial epithelial cells. Tissue clearing followed by laser scanning confocal microscopy enabled the three-dimensional visualization of intrapulmonary TexasRed dextran and the evaluation of its distribution at the alveolar scale without the preparation of thin tissue sections. These findings suggest that tissue-clearing techniques are useful for the evaluation of intrapulmonary distribution and development of pulmonary drug delivery systems.</p>
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 41 (1), 24-28, 2018
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679610984832
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- NII Article ID
- 130006301224
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 028739258
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- PubMed
- 29311480
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed