Role of endothelin-1 and interleukin-6 immunoreactivity in infarcted area of human stroke.

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  • ヒト脳梗塞巣におけるＩｎｔｅｒｌｅｕｋｉｎ‐６発現の意義について

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Abstract

Recent descriptions of the identification of the vasoconstrictor peptide, endothelin-1 (ET-1), and inflammatory interleukin-6 (IL-6) have attracted much attention and excited speculation regarding their possible pathological significance in cerebral infarction. The present study focused on the expression of ET-1 and IL-6 in the human stroke brain in relation to the healing process of occlusive cerebrovascular disease. Eight patients with occlusive cerebrovascular disease were employed. For immunohistochemical studies on ET-1 and IL-6, polyclonal anti-human ET-1 antibody (Peptide Institute Inc., Japan) and polyclonal rabit antibody against human IL-6 (Genzyme, Boston) were used, respectively. In the stroke brain at the chronic phase of cerebrovascular accident, the immunohistochemical method demonstrated simultaneous production of both ET-1 and IL-6 associated with astrocyte proliferation in serial sections derived from the boundary area. In addition, the endothelial cells demonstrated strong staining for ET-1 as well as for IL-6. The percentages of ET-1 expression in the astrocytes and endothelium were 87.6 ± 12.8% and 93.2 ± 7.8%, respectively. IL-6 also exhibited simultaneous expression in both the astrocytes (92.9 ± 5.4%) and endothelium (90.0 ± 9.9%). ET-1 and IL-6 can thus be produced by both astrocytes and endothelium, and act on them in intercellular reactions. The presence of IL-6 immunoreactivity in the infarcted area is functionally linked with ET-1. Our data indicate that, although the precise order remains unknown, a sequence of immunologic events may play a role in the pathophysiology of human stroke.

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