Complexes of Lactate Dehydrogenase Isoenzymes with Immunoglobulin G and Complement 3 in a Patient with Rheumatoid Arthritis

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  • Ishii Masao
    Second Department of Internal Medicine Kumamoto University Medical School
  • Matsuoka Yoshiko
    Second Department of Internal Medicine Kumamoto University Medical School
  • Ishii Toshinori
    Second Department of Internal Medicine Kumamoto University Medical School
  • Takatsuki Kiyoshi
    Second Department of Internal Medicine Kumamoto University Medical School

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  • 慢性関節リウマチ(RA)の経過中,血清中にLDH-IgG-C3複合体を証明しえた1例

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Complexes of lactate dehydrogenase with immunoglobulin (LDH-Ig) were detected in a case of RA. Circulating immune complex (CIC) and LDH activity were high in the serum of the patient.<br>By immunofixation, binding immunoglobulins were identified with IgG (κ) and IgG (λ). IgG (λ) disappeared soon, and only IgG (κ) existed for about 4 months. The two peaks of LDH activity were shown between 4S and 7S (Peak-S), 7S and 19S (Peak-F) by gel filtration on Sephadex G-200. By solid phase anti-C3 enzymelinked immunosorbent assay (ELISA), CIC level was high in Peak-F and low in Peak-S. In the sample of Peak-F fraction, LDH activity was high in the plates coated with anti-C3, anti IgG and anti κ antibody.<br>In conclusion, this is a first report that LDH-IgG binds with Complement and forms immune complexes. LDH activity was not affected by binding to IgG-C3.

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