書誌事項
- タイトル別名
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- Development of an LED-induced Fluorescence Analysis System Using a Compact Disk-type Microfluidic Device and Its Application to Enzyme-linked Immunosorbent Assay
- ネンカン トクシュウ ソラ コンパクトディスクガタ マイクロチップ オ モチイル LED ユウキ ケイ コウブンセキ システム ノ カイハツ ト コウソ メンエキ ソクテイホウ エ ノ オウヨウ
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説明
A novel LED-induced fluorescence analysis system using a CD-type microfluidic device was developed to miniaturize the elements of a complete analytical system. The determination of IgA in human saliva by ELISA using the developed system was evaluated. The solutions of the sample, washing reagent, enzyme-labeled anti-IgA, washing reagent and fluorescent substrate in the reservoirs on the CD-type microfluidic device were sequentially introduced into the separation and detection chamber, immobilized with an anti-IgA, by centrifugal force generated by rotation of the CD-type microfluidic device. The determination of IgA was performed by measuring the fluorescence intensity of the enzymatic product using a laboratory-made fluorescence detector with an LED and a CCD. The quantitative values of IgA obtained on the developed system were in excellent agreement with that on conventional ELISA using a 96-well microtiter plate. Since large-size and expensive peripheral equipment, such as a pump, valve, laser and microscope, are unnecessary, this system would be useful for on-site analysis, such as environmental monitoring, food safety testing and point-of-care testing.
収録刊行物
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- 分析化学
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分析化学 62 (2), 65-71, 2013
公益社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679636730880
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- NII論文ID
- 10031139092
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- NII書誌ID
- AN00222633
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- COI
- 1:CAS:528:DC%2BC3sXjsVehtL8%3D
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- NDL書誌ID
- 024291705
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- ISSN
- 05251931
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDLサーチ
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- 抄録ライセンスフラグ
- 使用不可
