パーソナル吸光度計を用いた鉄イオン及びタンパク質の定量分析

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書誌事項

タイトル別名
  • Quantitative Analyses of Iron Ion and Protein Using a Personal Spectroscope
  • アナリティカルレポート パーソナル吸光度計を用いた鉄イオン及びタンパク質の定量分析
  • アナリティカルレポート パーソナル キュウコウドケイ オ モチイタ テツ イオン オヨビ タンパクシツ ノ テイリョウ ブンセキ

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説明

Iron (II) ion and protein were determined with a personal spectroscope. The reaction of iron (II) with 1,10-phenanthroline to form a colored complex was used for iron (II) analysis. The personal spectroscope showed a linear plot in an analysis of the serial dilution of a standard iron (II) solution. The applicable concentration range was comparable to that of a usual spectroscope. Iron concentration in a health drink was successfully determined by this method. A determination of the composition of iron-phenanthroline complex was performed. We obtained a 1 : 3 metal : ligand ratio in the complex, the same as the theoretical value. Next, the protein concentration was determined by Bradford and BCA protein assay methods. The personal spectroscope showed linear plots from serial dilution of a standard BSA solution. The advantage of this spectroscope is a reduction of the amount of sample required (30 μL). The palm-size spectroscope can be controlled with a tablet so that it does not require large space for installation, and can be easily used. Therefore, the personal spectroscope is expected to be suitable for various purposes.

収録刊行物

  • 分析化学

    分析化学 65 (9), 533-537, 2016

    公益社団法人 日本分析化学会

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