Discrimination and Blend Ratio Estimation between Arabica and Robusta Coffee Species Using Direct Inlet Probe/Ion Attachment Ionization Mass Spectrometry

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  • ダイレクトインレットプローブ/イオン付着イオン化質量分析法を用いるコーヒー豆のアラビカ種及びロブスタ種の識別と配合比率の推定
  • ダイレクトインレットプローブ/イオン フチャク イオンカ シツリョウ ブンセキホウ オ モチイル コーヒーマメ ノ アラビカシュ オヨビ ロブスタシュ ノ シキベツ ト ハイゴウ ヒリツ ノ スイテイ

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Abstract

In this study, we examined a method to discriminate Coffea Arabica (Arabica) and Coffea canephora (Robusta). In order to explore the respective biomarkers, an ion attachment ionization mass spectrometer (IA-MS) equipped with a direct inlet probe (DIP) was used. It was possible to measure solid samples directly by DIP. Furthermore, in IA ionization mass spectra, because only quasi-molecular ion peaks are obtained, the mass spectra showed the characteristic pattern, which reflected the states in the sample. Therefore, easy screening analysis was possible. By using DIP/IA-MS, we measured nine kinds of roasted coffee beans. As a result, peaks estimated to be Kahweol palmitate and 16-O-methylcafestol palmitate were detected from Arabica and Robusta, respectively. In addition, this method offers an estimation of the mixed ratio of the Arabica and Robusta in not only roasted coffee beans milled, but extract with and without heat sterilization.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 63 (10), 825-830, 2014

    The Japan Society for Analytical Chemistry

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