Preparation of a Hydrophilic Stationary Phase Immobilizing an Acetylated Diallylamine–Acrylamide Copolymer and Its Retention Characteristics for Water-soluble Compounds

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  • KAMICHATANI Waka
    Products Planning & Development Dep., Research & Development Center, Nippon Filcon Co., LTD. College of Bioscience and Biotechnology, Chubu University
  • INOUE Yoshinori
    Products Planning & Development Dep., Research & Development Center, Nippon Filcon Co., LTD.
  • SAITO Mitsuru
    Products Planning & Development Dep., Research & Development Center, Nippon Filcon Co., LTD.
  • YAMAMOTO Atsushi
    College of Bioscience and Biotechnology, Chubu University

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Other Title
  • アセチル化ジアリルアミン—アクリルアミド共重合体を結合した親水性固定相の調製と水溶性化合物に対する保持特性
  • アセチル化ジアリルアミン-アクリルアミド共重合体を結合した親水性固定相の調製と水溶性化合物に対する保持特性
  • アセチルカ ジアリルアミン-アクリルアミド キョウ ジュウゴウタイ オ ケツゴウ シタ シン スイセイ コテイソウ ノ チョウセイ ト スイヨウセイ カゴウブツ ニ タイスル ホジ トクセイ

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Abstract

A new hydrophilic stationary phase (Ac-DAA resin) that was acetylated after immobilizing a diallylamine-acrylamide copolymer into a hydrophilic base resin was prepared. The retention characteristics for the water-soluble compounds on the Ac-DAA resin were evaluated by comparisons with the diallylamine-maleic acid copolymer immobilized stationary phase (DAM resin). The copolymer-immobilizing content of the Ac-DAA resin was approximately 2.5-times higher than that of the DAM resin, but the contents of holding-water and hydration water were almost the same. Water-soluble compounds, such as nucleobase, nucleoside and glucoside, were clearly retained on both stationary phases by hydrophilic interaction. However, for the nucleobases, the specific affinity, which was estimated to be a hydrogen associativity, was observed in the Ac-DAA resin. Further, the strength of the electrostatic interaction in both stationary phases was compared using ionic water-soluble compounds as analytes. The electrostatic interaction of the Ac-DAA resin was stronger than that of the DAM resin. However, by adjusting the buffer solution pH of the mobile phase above 8, the electrostatic interaction of the Ac-DAA resin could be suppressed without a decrease of retention based on the hydrophilic interaction. It was concluded that the Ac-DAA resin was effective for the separation of water-soluble compounds including ionic compounds by employing the mobile phase adjusted with an appropriate pH.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 61 (7), 613-619, 2012

    The Japan Society for Analytical Chemistry

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