Fluorescence Imaging of Virus-infected Cells with a Sialidase Imaging Probe

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  • TAKAHASHI Tadanobu
    Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka
  • KUREBAYASHI Yuuki
    Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka
  • OTSUBO Tadamune
    Department of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International University
  • IKEDA Kiyoshi
    Department of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International University
  • MINAMI Akira
    Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka
  • SUZUKI Takashi
    Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka

Bibliographic Information

Other Title
  • シアリダーゼを利用したウイルス感染細胞の蛍光イメージング
  • シアリダーゼ オ リヨウ シタ ウイルス カンセン サイボウ ノ ケイコウ イメージング

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Abstract

Influenza A and B viruses and some paramyxoviruses possess an enzyme "sialidase" that cleavages terminal sialic acid from saccharides. These viral sialidases are highly expressed on infected cells. Our developed sialidase imaging probe, "BTP3-Neu5Ac", enables histochemical fluorescence staining of sialidase activity. BTP3-Neu5Ac was able to perform easy and speedy fluorescence imaging of these infected cells, with no needs of anti-virus antibody and cell fixation. In addition, combination use of anti-influenza drugs (sialidase inhibitors) and BTP3-Neu5Ac resulted in selective fluorescence imaging of drug-resistant virus-infected cells and high-efficiency isolation of drug-resistant virus. A new tool for fluorescence imaging of viral sialidase activity is described in this review.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 65 (12), 689-701, 2016

    The Japan Society for Analytical Chemistry

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