書誌事項
- タイトル別名
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- Development of a Micro RNA Extraction Chip from Human Tumor Cells
- マイクロ チュウシュツジョウ ニ ヨル ヒト シュヨウ サイボウ ユライ ノ マイクロ RNA チュウシュツホウ
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抄録
The importance of micro RNA analysis has been significantly increasing because the role of micro RNA in the human body has been gradually revealed. Despite its importance, the analysis has suffered from several troublesome pretreatments, which hamper any easy analysis. Therefore, an easy and high-throughput pretreatment method has been demanded. In this paper, we focused on the great advantages of microchip pretreatments over conventional manual pretreatments and developed a microchip for micro RNA extraction. To simplify microchip fabrication, we adopted poly(dimethyl siloxane) (PDMS) microchip and a silica membrane, which has rolls in RNA extraction fields. With silica membranes and the adhesion nature of PDMS, we could easily fabricate RNA extraction fields in the microchip. With this microchip, we successfully extracted micro RNA from cancer tumor cells. Though this is a preliminary experiment, and still has many improvement points, our device is expected to be applied for easy and fast micro RNA extraction from biological samples.
収録刊行物
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- 分析化学
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分析化学 64 (1), 9-13, 2015
公益社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679638197888
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- NII論文ID
- 130004732535
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- NII書誌ID
- AN00222633
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- HANDLE
- 2115/71683
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- NDL書誌ID
- 026052046
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- ISSN
- 05251931
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可