Examination of Reliability and Correction Method for Quantitative Estimation of Carbon Monoxide Hemoglobin in Degenerated Blood Using Different Calculation Method of Regression Analysis (DCR) and Principal Component Analysis (PCA)

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  • 改重回帰分析（DCR）と主成分分析（PCA）による変性した血液中の一酸化炭素ヘモグロビン飽和度の定量結果の信頼性と補正方法の検討
  • カイジュウ カイキ ブンセキ(DCR)ト シュセイブン ブンセキ(PCA)ニ ヨル ヘンセイ シタ ケツエキ チュウ ノ イッサンカ タンソ ヘモグロビン ホウワド ノ テイリョウ ケッカ ノ シンライセイ ト ホセイ ホウホウ ノ ケントウ

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Abstract

The determination of carbon monoxide hemoglobin (CO-Hb%) in blood by different calculation methods involving regression analysis (DCR) and principal component score (PCA) was carried out using 0% and 100% of CO-Hb% in blood. However, both hemoglobin and carbon monoxide hemoglobin in blood are degenerated by heat or putrefaction. As a result, the correct CO-Hb% in blood is not obtained, and any judgment of the cause of death may be mistaken. Thus, the degree of degeneration of blood was examined based on regression statistics (R2), the sum of the coefficient in the multiple-regression equation, and the sum of the third principal component score. As a result of degenerations, the values of R2 and the sum of the coefficients in multiple regression equation decreased, and the sum of the third principal-component score is increased. Consequently, the presumption of the degree of degeneration in blood was attained from the change of these values. Also, a check of the cause of degeneration was attained from the third principal-component residual spectrum of the sample blood. Furthermore, the correct CO-Hb% in the sample blood was calculated from the determination value of CO-Hb% and the absorbance of the third principal component of 533 or 570 nm.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 62 (5), 423-429, 2013

    The Japan Society for Analytical Chemistry

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