Determination of 7 Kinds of Sulfa Drugs in a Pork Sample by HPLC Using a Biphenyl Core-shell Stationary-phase Column

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  • ビフェニルコアシェルカラムを用いるHPLCによる豚肉中のサルファ剤7種の定量
  • アナリティカルレポート ビフェニルコアシェルカラムを用いるHPLCによる豚肉中のサルファ剤7種の定量
  • アナリティカルレポート ビフェニルコアシェルカラム オ モチイル HPLC ニ ヨル ブタニク チュウ ノ サルファザイ 7シュ ノ テイリョウ

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Abstract

Sulfa drugs are very stable and highly lipophilic. They are easily accumulated in the human body and in edible agricultural products. Recently, in 2006, the Ministry of Health in Japan proposed the Positive List System for the regulation of agricultural chemical residues in foods. In this study, the determination of seven kinds of sulfa drugs [Sulfisoxazole (SIX), Sulfadiazine (SDZ), Sulfamethoxazole (SMXZ), Sulfamethoxazole (SMMX), Sulfamerazine (SMR), Sulfamerazine (SMPD), Sulfadimidine (SDD)] in a pork sample by high-performance liquid chromatography (HPLC) with an ultraviolet detector (UV) on a biphenyl core-shell stationary phase column was developed. This analytical method provides high linearity of the calibration curve as well as repeatability. The correlation coefficients of the working curve of calibration were estimated to be from 0.9991 to 0.9999 for seven kinds of sulfa drugs in the concentration range from 1 mg L−1 to 10 mg L−1. The limits of detection (LOD) calculated on 3σ at 1 mg L−1 were 0.017 mg L−1 for SIX, 0.059 mg L−1 for SDZ, 0.045 mg L−1 for SMXZ, 0.035 mg L−1 for SMMX, 0.068 mg L−1 for SMR, 0.016 mg L−1 for SMPD, and 0.056 mg L−1 for SDD. The limits of quantification (LOQ), calculated on 10σ at 1 mg L−1, were 0.057 mg L−1 for SIX, 0.197 mg L−1 for SDZ, 0.149 mg L−1 for SMXZ, 0.115 mg L−1 for SMMX, 0.227 mg L−1 for SMR, 0.054 mg L−1 for SMPD, and 0.186 mg L−1 for SDD. Good results, the recoveries used by acetonitrile were 77.1–97.6% and the relative standard deviations (RSD) of peak area were less than 1% (N = 3), were obtained in spike tests in a pork sample by the QuEChERS method. This proposed method could be successfully applied to the determination of seven kinds of sulfa drugs in a pork sample.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 65 (2), 87-92, 2016

    The Japan Society for Analytical Chemistry

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