比色分析による混合製剤中のアミノプロピロンの定量

DOI Web Site Web Site

書誌事項

タイトル別名
  • Spectrophotometric determination of aminopropyrone in pharmaceutical preparations
  • ヒショク ブンセキ ニ ヨル コンゴウ セイザイ チュウ ノ アミノプロピロン ノ テイリョウ
  • Studies on analysis of mixed pharmaceutical preparations. IX
  • 混合製剤の分析に関する研究(第9報)

この論文をさがす

説明

Aminopropyrone (I) react with CuSO4 and pyridine to give a light-green color (Cu complex) with λmax absorption at 410 mμ.<BR>A new spectrophotometric method for (I) based on this color reaction was established, and applied to the determination of (I) in pharmaceutical preparations.<BR>The influences of 48 compounds on the determination were investigated, among which those by sulpyrine, N-methylaminoantipyrine, salicylates, theophylline, barbiturates, amino acids, ascorbic acid, sodium citrate etc. could be eliminated by chloroform extraction in an alkaline medium.<BR>The recommended analytical procedure was as follows. Take the sample containing 50 mg of (I) in a separatory funnel. Add 20 ml of water and 10 ml of 0.1N NaOH, and extract 5 times with 30 ml portions of chloroform. Combine the chloroform extracts and evaporate on a water bath.<BR>Dissolve the residue in 50 ml of water. Transfer the solution to a 100 ml volumetric flask, and make up to volume exactry with water.<BR>Transfer a 5 ml aliquot into a 30 ml glass-stoppered test tube, and add 5 ml of 0.1% CuSO4-pyridine solution. Add water to make up to 25 ml, and measure the absorbance at 410 mμ (ET) against water.<BR>At the same time, the absorbance of the standard solution (ES) is determined and the amount of (I) is calculated by ET /ES.

収録刊行物

  • 分析化学

    分析化学 17 (5), 555-559, 1968

    公益社団法人 日本分析化学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ