Determination of Micro Amount of Hydrogen Peroxide by a Zero-emission FIA System with Immobilized Peroxidase Enzyme Reactor Column

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  • SAKURAGAWA Akio
    Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University
  • YOSHIKAWA Kenji
    Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University
  • ISHIDUKA Shinnosuke
    Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University

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Other Title
  • 固定化ペルオキシダーゼ反応カラムを用いるゼロエミッションフローインジェクション法による微量過酸化水素の定量
  • コテイカ ペルオキシダーゼ ハンノウ カラム オ モチイル ゼロエミッションフローインジェクションホウ ニ ヨル ビリョウ カサンカスイソ ノ テイリョウ

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Abstract

A zero-emission flow injection analysis (FIA) system with an immobilized peroxidase enzyme reactor column (POD-IE) used for the determination of a micro amount of hydrogen peroxide has been developed. A FIA system composed of multiple flow channels was reconstructed to be a mono flow channel; the HPPA dimer generated by the reaction among hydrogen peroxide, horseradish peroxidase and 3-(p-hydroxyphenyl)-propionic acid (HPPA) was utilized as a fluorometric indicator. After measuring the fluorescence intensity of the HPPA dimmer, the flow stream passes through an anion exchange resin column (packed Waters: sep-pak Accell plus QMA). The HPPA dimer and only non-reacted HPPA reagent are removed, and the stream is circulated. A solution containing hydrogen peroxide was injected into the FIA system in previous work. In the present work, it was injected into a solution mixing HPPA and a sample solution containing hydrogen peroxide, for such a purpose as reducing the amounts of reagents used. The correlation coefficient of the FIA system was more than 0.998 in the linear range of the calibration curve (0 to 0.070 ppm). The relative standard deviation at 0.040 ppm of hydrogen peroxide was 6.86% (n = 7). After only effects of coexisting substances and ions thought to be contained in rain-water were examined, it was applied to the determination of hydrogen peroxide in the rain-water as practical samples.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 63 (7), 603-607, 2014

    The Japan Society for Analytical Chemistry

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