Improved high performance liquid chromatography determination of hippuric acid and methylhippuric acid isomers in urine.
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- TAKEUCHI Yukiko
- Center of Occupational Medicine, Tokyo Labor Accident Hospital
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- SAKAI Tadashi
- Center of Occupational Medicine, Tokyo Labor Accident Hospital
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- IKEYA Yumiko
- Center of Occupational Medicine, Tokyo Labor Accident Hospital
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- ARAKI Takaharu
- Center of Occupational Medicine, Tokyo Labor Accident Hospital
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- USHIO Koichi
- Center of Occupational Medicine, Tokyo Labor Accident Hospital
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- YANAGIHARA Susumu
- Center of Occupational Medicine, Tokyo Labor Accident Hospital
Bibliographic Information
- Other Title
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- 高速液体クロマトグラフによる尿中馬尿酸およびメチル馬尿酸の分離定量法についての検討
- コウソク エキタイ クロマトグラフ ニ ヨル ニョウチュウ バニョウサン オヨ
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Abstract
A study was made on the effect of detection wavelength and separation mode of HPLC on determination of urinary hippuric acid (HA) and three isomers of methylhippuric acid (MHA). The interference of other constituents of urine in the determination was effectively decreased by detection at a short wavelength of 227.6nm. Meta and para MHAs were separated by the addition of β-cyclodextrine to the mobile phase. Four metabolites were successfully separated from other components of urine by the combination of ODS-silica packed-column and mobile phase (method F). The detection limits were found to be 50 and 5mg/l for HA and MHAs, respectively. MHAs could not be detected in the non-exposed subjects. Average levels (±SD) of HA in non-exposed males and females were 272.2 (±210.8) and 393.0 (±269.8)mg/l, respectively. The urinary levels of HA in females were significantly higher than those in males.
Journal
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- Sangyo Igaku
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Sangyo Igaku 28 (6), 455-461, 1986
Japan Society for Occupational Health
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Keywords
Details 詳細情報について
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- CRID
- 1390282679640529280
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- NII Article ID
- 110006645060
- 130003505171
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- NII Book ID
- AN00098250
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- COI
- 1:CAS:528:DyaL2sXktVajtL8%3D
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- ISSN
- 18811302
- 00471879
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- NDL BIB ID
- 3123572
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- PubMed
- 3586389
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed