細胞の解凍過程におけるミク口挙動と障害

DOI Web Site 被引用文献2件 参考文献13件

書誌事項

タイトル別名
  • Micro-behavior and Injury of Biological Cell during Thawing Process
  • 細胞の解凍過程におけるミクロ挙動と障害
  • サイボウ ノ カイトウ カテイ ニ オケル ミクロ キョドウ ト ショウガイ

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This study has been conducted to pursue the relation between microscale behavior and the injury of biological cell during freezing and thawing. As a sample of biological cells, protoplasts isolated from cultured wheat cells were selectively used. As the results of microscopic observation using a cold stage whose cooling and heating velocities were controlled, the recovery of cell by water influx due to osmotic pressure difference, and the fusion of intracellular ice were clarified with heating velocity. It was found that the osmotic stress acting on the ce11 membrane causes the thawing injuries connecting with swell and rupture of cell. The survival of cells was also inspected by dye-exclusion test using Evans Blue. The results suggested rapid temperature-rising is more harmful for slowly-frozen cell.

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