Alcohol is an oxidative stressor for gastric epithelial cells: detection of superoxide in living cells
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- Tamura Masato
- Faculty of Medicine, University of Tsukuba
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- Matsui Hirofumi
- Faculty of Medicine, University of Tsukuba
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- Kaneko Tsuyoshi
- Faculty of Medicine, University of Tsukuba
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- Hyodo Ichinosuke
- Faculty of Medicine, University of Tsukuba
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Description
Alcohol/ethanol has been reported to derived necrosis and apoptosis with an oxidative stress in gastric mucosal cells. However the clear evidence for reactive oxygen species (ROS) generation by alcohol in gastric cells in vitro is none. In this study, we elucidated ethanol is an oxidative stress inducer on rat gastric epithelial cells by electron paramagnetic resonance measurement in living cells. We also confirmed whether ethanol-induced cellular ROS was derived from mitochondria or not. The results of cellular ROS determination showed that an increment of cellular ROS was shown for 15 min from exposing 1% (v/v) ethanol. Lipid peroxidation in cellular membrane also induced by 1% ethanol and the tendency is same in the results of cellular ROS determination. JC-1 stained showed the decrement of mitochondrial membrane potential. Additionally the localization of cellular ROS coincided with mitochondria. These results indicated that ethanol is not merely a necrotizing factor for gastric epithelial cells, but also an oxidative stress inducer via injured mitochondria.
Journal
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 53 (2), 75-80, 2013
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Details 詳細情報について
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- CRID
- 1390282679649539584
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- NII Article ID
- 130004466720
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- NII Book ID
- AA10710201
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- COI
- 1:CAS:528:DC%2BC3sXhs1agsrfK
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- ISSN
- 18805086
- 09120009
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- HANDLE
- 2241/120066
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- PubMed
- 24062603
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- Crossref
- PubMed
- CiNii Articles
- KAKEN
- OpenAIRE
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- Abstract License Flag
- Disallowed