Detection of Nε-(hexanoyl)lysine in the tropomyosin 1 protein in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat gastric cancer cells
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- Okada Hitomi
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Naito Yuji
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Takagi Tomohisa
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Takaoka Megumi
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Oya-Ito Tomoko
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Fukumoto Kohei
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Uchiyama Kazuhiko
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Handa Osamu
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Kokura Satoshi
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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- Nagano Yumiko
- Department of Gastroenterology, Institute of Clinical Medicine, University of Tsukuba
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- Matsui Hirofumi
- Department of Gastroenterology, Institute of Clinical Medicine, University of Tsukuba
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- Kato Yoji
- School of Human Science and Environment, University of Hyogo
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- Osawa Toshihiko
- Department of Nutritional Science, Aichi Gakuin University
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- Yoshikawa Toshikazu
- Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
書誌事項
- タイトル別名
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- Detection of N.EPSILON.-(hexanoyl)lysine in the tropomyosin 1 protein in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat gastric cancer cells
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説明
Nε-(Hexanoyl)lysine, formed by the reaction of lysine with n-6 lipid hydroperoxide, is a lipid peroxidation marker during the initial stage of oxidative stress. The aim of the present study is to indentify Nε-(hexanoyl)lysine-modified proteins in neoplastic transformed gastric mucosal cells by N-methyl-N’-nitro-N-nitrosoguanidine, and to compare the levels of these proteins between gastric mucosal cells and normal gastric cells. Much greater fluorescence of 2-[6-(4'-hydroxy)phenoxyl-3H-xanthen-3-on-9-yl]benzoic acid, an index of the intracellular levels of reactive oxygen species, was observed for gastric mucosal cells compared to normal gastric cells. Nε-(Hexanoyl)lysine-modified proteins were detected by SDS-PAGE or two-dimensional electrophoresis and Western blotting using anti-Nε-(hexanoyl)lysine polyclonal antibody, and a protein band of between 30–40 kDa was clearly increased in gastric mucosal cells compared to normal gastric cells. Two Nε-(hexanoyl)lysine-modified protein spots in gastric mucosal cells were identified as the tropomyosin 1 protein by mass spectrometry using a MASCOT search. The existence of Nε-(hexanoyl)lysine modification in tropomyosin 1 was confirmed by Western blotting of SDS-PAGE-separated or two-dimensional electrophoresis-separated proteins as well as by the immunoprecipitation with anti-tropomyosin 1 antibody. These data indicate that Nε-(hexanoyl)lysine modification of tropomyosin 1 may be related to neoplastic transformation by N-methyl-N'-nitro-N-nitrosoguanidine in gastric epithelial cells.<br>
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ
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Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ 50 (1), 47-52, 2011
一般社団法人 日本酸化ストレス学会