Protein adsorption dialysis membrane (ethylene vinyl alcohol membrane: EVAL) with an applied negative charge.

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  • 陰性荷電を付加した透析膜（ｅｔｈｙｌｅｎｅ　ｖｉｎｙｌ　ａｌｃｏｈｏｌ膜：ＥＶＡＬ）における蛋白の吸着に関する検討

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Heparan sulfate proteoglycan (HS-PG), which forms the charge barrier of the glomerular basement membrane (GBM), is considered to play an important role in maintaining the effective filtration of solutes since it preserves the hydrophilic character of the GBM. It does this by promoting water retention among the sulfonate groups and preventing clogging of the GBM under physiological conditions. Therefore, we studied the relation between negative electric charge and adsorption by determining the adsorptivity of a dialysis membrane, with an applied sulfonate group, for various proteins.<br>We found that the negatively charged membrane adsorbed positively charged proteins whose isoelectric points were more than 9.0. Furthermore, the negatively charged membrane achieved greater adsorption of proteins with higher positive charges. The degrees of such adsorption were therefore considered to be largely dependent upon the isoelectric points of proteins in cases in which the molecular weights of the proteins were nearly the same.

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