Effect of prostaglandin E<SUB>1</SUB> on bovine periodontal ligament

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  • ウシ歯根膜におけるＰｒｏｓｔａｇｌａｎｄｉｎ　Ｅ１の影響　　Ｓｕｃｃｉｎａｔｅ‐１．４‐｀１４´Ｃ由来の有機酸代謝及び新生高分子について
  • Succinate-1, 4-<SUP>14</SUP>C由来の有機酸代謝及び新生高分子について

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Abstract

Prostaglandins have been recognized recently as one of the important chemical mediators in periodontal disease. The present study is aimed at studying the metabolic alteration in periodontal tissues mediated by prostaglandins.<BR>The effect of prostaglandin E1 (5 to 200ng/ml) on bovine periodontal ligament cells was investigated by using the short term incubation system with succinate-1, 4-14C as the radioactive tracer. The extracellular medium was analysed for TCA cycle intermediates and synthesized macromolecules.<BR>Organic acid analysis showed that the synthetic ratios of the pyruvate: malate, and citrate:α ketoglutarate were altered by PGE1. Especially, α-ketoglutarate: isocitrate ratio increased markedly with 5ng PGE1/ml.<BR>Macromolecular sythesis was inhibited at low concentration and accelerated at high concentration of PGE1. The products over 65 K dalton decreased about 1/3 with 5ng PGE1/ml, and increased with 200ng PGE1/ml, but the substances of 32-65 K dalton increased about 1/4 with 5ng PGE1/ml and decreased 15.6% with 200ng PGE1/ml.<BR>It was found that the synthesized macromolecules of 59 K dalton estimated on the slab SDS polyacrylamide gel electrophoresis was remarkably induced by the low concentration of PGE1 (5ng/ml).<BR>These results demonstrated that PGE1 is one of the mediators of periodontal tissue cells alterated their organic acid metabolism and macromolecular synthesis.

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