Mechanism for synergistic effect of bacterial lipopolysaccharide and concanavalin A on DNA synthesis in mouse splenic lymphocytes.

Nitta Toshimasa, Okumura Seiichi, Nakano Masayasu

doi:10.2330/joralbiosci1965.28.350

Bacterial lipopolysaccharide (LPS) was not mitogenic for murine T lymphocytes, but was capable of enhancing [³H] thymidine uptake of T lymphocytes stimulated by concanavalin A (Con A) in the presence of B lymphocytes or cmarophages (Mφ) in vitro. The mechanisms for the synergy of LPS and Con A on the proliferation of murine splenic lymphocytes and the relation of interleukin (IL)-production to the synergy were studied. The proliferative cells in the synergy were Lyt-1 positive T cells. A mixed cell population of T cells and B cells or Mφ produced active factor (s) after costimulation with Con A and LPS, and the factor (s) enhanced the proliferation of Con A-activated T cell population. The supernatants of spleen cell cultures which had been cultured with Con A and LPS contained greater amounts of IL 1 and IL 2 than those with LPS or Con A alone. Addition of exogenous IL 2, but not IL 1, into the Con A-activated T cell population increased their proliferative responses. These results indicate that LPS has the ability to enhance the production of IL 2 from Con A-activated T cells, and the IL 2 helps the proliferation of Con A-stimulated T cells. Thus, the experimental results strongly suggest that the synergistic effect of LPS and Con A on lymphocyte proliferation is mainly due to the action of IL 2.

Mechanism for synergistic effect of bacterial lipopolysaccharide and concanavalin A on DNA synthesis in mouse splenic lymphocytes.

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