Cytochemical localization of peroxidase in the acinar cells of new-born and adult Mongolian gerbil submandibular gland.
-
- Takano Kunio
- Department of Oral Histology, Nagasaki University School of Dentistry
-
- Muraki Kayoko
- Department of Oral Histology, Nagasaki University School of Dentistry
-
- Miyazaki Toshihiro
- Department of Oral Histology, Nagasaki University School of Dentistry
Bibliographic Information
- Other Title
-
- スナネズミがっ下せんにおけるペルオキシダーゼの細胞化学的局在について
Abstract
The cytochemical localization of endogenous peroxidase was demonstrated in the acinar cells of new-born and adult Mongolian gerbil submandibular glands in order to investigate a peroxidase synthetic pathway in an acinar cell. The secretory granules in the acinar cells were seen to be packed with dense substances. The more densely stained part of the granule showed a strong peroxidase activity. The present study focussed on the Golgi area to demonstrate the transportation of the enzyme protein of peroxidase from the rough endoplasmic reticulum to the secretory granule in the acinar cell, by means of the Graham and Karnovsky DAB method (1966). The transport vesicles formed by budding from the endoplasmic reticulum showed evidence of peroxidase activity. Reaction products were also seen in the cisternae of the endoplasmic reticulum, the nuclear envelope, immature secretory granules and in the condensing vacuoles situated away from the Golgi stack. A positive DAB reaction, however, was not found in the Golgi cisternae and in the adjoining immature condensing vacuoles. The peroxidase enzyme in the acinar cells of Mongolian gerbil submandibular gland therefore appears to be tr ansported from the rough endoplasmic reticulum to the condensing vacuoles via transport vesicles in the periphery of the Golgi complex.
Journal
-
- Japanese Journal of Oral Biology
-
Japanese Journal of Oral Biology 27 (2), 534-548, 1985
Japanese Association for Oral Biology
- Tweet
Details 詳細情報について
-
- CRID
- 1390282679660217216
-
- NII Article ID
- 130004001353
-
- ISSN
- 03850137
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed