Serological identification of S. sanguis type I and serological relationship between S. sanguis type I and streptococcus group H

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  • TOMISAWA Mannosuke
    Department of Oral Microbiology, School of Dentistry, Iwate medical University
  • HONDA Hisako
    Department of Oral Microbiology, School of Dentistry, Iwate medical University
  • HIRATA Yoshiko
    Department of Oral Microbiology, School of Dentistry, Iwate medical University
  • TAJIKA Shihoko
    Department of Oral Microbiology, School of Dentistry, Iwate medical University

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  • S.sanguis type Iの血清学的同定およびレンサ球菌H群とS.sanguis type I

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Serological studies were carried out with isolated and reference strains. Extract was prepared according to the method of Rantz and Randall, and antiserum was prepared by immunizing rabbit with formalin-killed organisms.<BR>Precipitin reaction (ring prove) was observated for 2 hours at room temperature.<BR>The antigen titer of the strain belonging to S. sanguis type I is almost constant, 1: 128-1: 256, while the antibody titer is not constant. In the serological experiment, antiserum with as high antibody titer as possible was used.<BR>Among the extracts in twofold dilution made of streptococci isolated from the dental plaque, precipitin reaction were carried out only in 39 strains reacting intensely with S. sanguis type I antiserum alone, and 35 strains of these were identified as S. sanguis type I. The biological properties of these strains resemble those of S. sanguis type I.<BR>Strains Challis and M-5 were found to be serologically most similar to S. sanguis type I-II ATCC 10558. Strain 8144, 12396, F 90 A and Wicky in the group H streptococcus gave different results.

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