アスペルギルス症の１例　真菌用蛍光染色液を用いた重染色の試み

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  • A case of aspergillosis. Application of double staining with fluorescence dye for fungi.
  • Application of double staining with fluorescencedye for fungi
  • 真菌用蛍光染色液を用いた重染色の試み

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We report an autopsy case of Aspergillus brain abscess that had extended from Aspergillus sinusitis, and attempted to detect the fungus in cytology specimens byFungiflora Y® and Papanicolaou double staining. An 80-year-old man was found to have right orbital and sphenoidal aspergillosis. Treatment with antifungal agents was ineffective, and a brain abscess was detected by magnetic resonance imaging seven months later. The patient's condition deteriorated and he died. Pus from the brain abscess was used to makes smear and Fungiflora Y®-Papanicolaou double staining was performed. It was difficult to detect the fungi on screening with the ×10 objective lens of light microscope because of their faint color. However, under a fluorescence microscope the fungal hyphae were apple green and it was very easy to detect them. Thus, Fungiflora Y®-Papanicolaou double staining seems very useful for detecting fungal hyphae on smear cytology.

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