Immunocytochemical study of Papanicolaou-stained anterior lobe cells of the pituitary with application to pituitary adenoma.

DOI 1 Citations
  • MORIWAKI Tomoko
    Cytology, Pathology Section, Clinical Laboratory, St. Marianna University Hospital
  • HIRANO Mieko
    Cytology, Pathology Section, Clinical Laboratory, St. Marianna University Hospital
  • HOSHIKAWA Sakiko
    Cytology, Pathology Section, Clinical Laboratory, St. Marianna University Hospital
  • TADOKORO Mamoru
    Department of Pathology, St. Marianna University School of Medicine
  • YASHIRO Takashi
    Ist Department of Anatomy, St. Marianna University School of Medicine
  • ARAI Motonaka
    Ist Department of Anatomy, St. Marianna University School of Medicine
  • SUZUKI Takuro
    Ist Department of Anatomy, St. Marianna University School of Medicine
  • USHIGOME Shinichiro
    Ist Department of Pathology, The Jikei University School of Medicine

Bibliographic Information

Other Title
  • Ｐａｐａｎｉｃｏｌａｏｕ染色された下垂体前葉細胞の免疫組織化学的検索　　下垂体腺腫の細胞診への応用
  • 下垂体腺腫の細胞診への応用

Search this article

Abstract

In order to examine whether cytologic examination of pituitary adenoma might enable evaluation of hormonal function of tumor cells, an attempt was made to clarify how hormone-secreting cells and non-granulated cells of the pituitary react to the various dyes in the Papanicolaou stain such as hematoxylin, orange. G, eosin Y, light green and Bismarck brown. Each cells was comparatively identified by immunocytochemistry using antisera to GH (growth hormone), ACTH (adrenocorticotrophic hormone), TSH (thyroid stimulating hormone), LH (luteiniging hormone), PRL (prolactin) and S-100 protein.<BR>GH cells appeared to contain dense compound granules of orange G and eosin Y. ACTH cells showed dense dark brown granules in the cytoplasm. TSH and LH cells disclosed light green cytoplasm with scattered, hematoxylinstained granules. S-100 proteinpositive cells (non-granulated cells) tended to stain with light green, but faintly. PRL cells were devided into three types?(1) only light green stained cells, (2) light green cytoplasm with scattered compound granules of eosin Y and orange G, and (3) cells with dense compound granules of eosin Y and orange G.<BR>Judging from these stain characteristics, the hypothesis of evaluation of the hormone-secreting function of tumor cells seemed possible to some extent by routine cytologic examination of pituitary adenoma.

Journal

Citations (1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top